Outcome measures included BMI, waist circumference, blood pressure, insulin sensitivity, plasma glucose, plasma insulin, triglycerides, HDL, LDL, demographic information, perceived stress (PSS), dietary information using a food frequency questionnaire (2005 Block questionnaire), physical activity, quality of life (SF-36), depression (CES-D), and a self-rating of overall health using a Likert scale. This evidence concerns the gene INS and depressive symptom measurement.