Outcome measures included blood pressure, lipoprotein profile, insulin resistance using the homeostasis model assessment (HOMA), endothelial function using the brachial artery reactivity test (BART), heart rate variability (HRV), medical history, body mass index (BMI), hostility, depression, trait anxiety, trait anger, stress, plasma cholesterol, triglycerides, HDL cholesterol, plasma glucose, plasma insulin, and high-sensitivity C-reactive protein (hs-CRP). This evidence concerns the gene INS and major depressive disorder.