TP53 and carcinoma: Expression of p53 protein was observed in the nucleus of carcinoma cells (Figure 3A and B), and the levels were variable: grade − in 47 (49.5%) cases, grade 1+ in 15 (15.8%) cases, grade 2+ in 17 (17.9%) cases, and grade 3+ in 16 (16.8%) cases of 95 CRCs (Table 1).