The present study demonstrated that, in in vitro study, donepezil inhibited MMP-9 in LPS-treated macrophages (Figures 1, 2, 3, 4) and that, in in vivo study, donepezil attenuated the inflammatory cell infiltration into the infarcted myocardium and decreased the expression and the enzymatic activity of MMP-9 at the infarct area (Figure 7), thereby reduced the risk of cardiac rupture during the acute phase of MI (Table 3). This evidence concerns the gene MMP9 and myocardial infarction.