Consistent with previous studies, we found that T24 cells originating from the highest grade III TCC expressed the lowest level of E-Cadherin and Cytokeratin, and the highest level of Cox-2, Vimentin and EMT-transcriptional factors (SNAIL, Slug, Twist and Zeb 1) mRNA among the three human bladder cancer cell lines (Figure 2). Here, TWIST1 is linked to tarsal-carpal coalition syndrome.