Consistent with previous studies, we found that T24 cells originating from the highest grade III TCC expressed the lowest level of E-Cadherin and Cytokeratin, and the highest level of Cox-2, Vimentin and EMT-transcriptional factors (SNAIL, Slug, Twist and Zeb 1) mRNA among the three human bladder cancer cell lines (Figure 2). The gene discussed is TWIST1; the disease is urinary bladder cancer.