Transfection of miR-34a precursor downregulated mRNA expression of the AXL gene in A549 and NCI-H1299 NSCLC cells by more than 70% (change of delta Ct by more than 1.7), whereas the mRNA expression of the AXL gene in NCI-H82 and NCI-N592 SCLC cells was too low to be detected by real-time PCR assay (Figure 3B). The gene discussed is AXL; the disease is small cell lung carcinoma.