Takahashi et al. utilized a rat model of post-MI heart failure and an experimental protocol similar to the one adopted in the present work, i.e. three weeks after coronary ligation HMGB1 was injected intramyocardially at a dose that, adjusted by weight, was similar to the dose injected in our protocol; thereafter mice were followed for four additional weeks. Here, HMGB1 is linked to myocardial infarction.