Also, overexpression of the DNA methyltransferase, DNMT3B, was identified in lung and breast cancer cell lines (Beaulieu et al, 2002) and depletion of DNMT3B expression in breast cancer cell line was shown to activate the methylated RASSF1 (Wang et al, 2006); therefore, we analysed the expression of these factors by qRT-PCR. The gene discussed is RASSF1; the disease is breast carcinoma.