In these studies [60,91,129], the two subtypes are hepatic stem cell like HCC (HpSC-HCC; EpCAM+AFP+) and mature hepatocyte like HCC (MH-HCC; EpCAM-AFP-), where HpSC-HCC cells are hepatic CSCs with the ability to self-renew, differentiate and initiate aggressive tumors in vivo [60]. This evidence concerns the gene AFP and hepatocellular carcinoma.