To further determine whether TLR4 played a potential role consequent to the inhibitory effect of APS on CD4+CD25+Tregs, we investigated changes in intranuclear Foxp3 protein, Foxp3 mRNA expressions, and IL-10 secretion of APS-Tregs with anti-TLR4 and isotype of TLR4 on PBD 3 and 7. The gene discussed is CD4; the disease is autoimmune polyendocrinopathy.