After APS stimulation, CD4+CD25+Tregs were separated from APS+CD4+CD25+Tregs, APS+anti-TLR4+CD4+CD25+Tregs and APS+TLR4-isotype+CD4+CD25+Tregs mixed culture, and subsequently, used to administrate CD4+CD25+ Tregs + CD4+ T cells MLR. The gene discussed is TLR4; the disease is autoimmune polyendocrinopathy.