This issue is particularly relevant in onco-pathology for the evaluation of factors predicting responsiveness to therapeutic treatments, and thus, fixation in phosphate buffered formalin (PBF) of breast cancer tissue blocks for no less than 6 and no more than 48 hours is now required in order to guarantee an optimal evaluation of Estrogen (ER) and Progesterone Receptors (PgR) and HER2 expression [4], [5]. This evidence concerns the gene ESR1 and breast carcinoma.