HIPK2 and thyroid gland carcinoma: In particular, we analyzed HIPK2 protein expression by IHC in thyroid histological slides, HIPK2 mRNA expression by Real Time RT-PCR on total RNA extracted from frozen thyroid tissues samples, and genetic loss at HIPK2 locus, by LOH analysis in thyroid cancer cells, stained with Gal-3, and retrieved by LCM.