In the present study, large scale immunohistochemical tissue array analysis was performed, and the cytoplasmic pFOXO1 expression in tumor cells was found to be positively correlated with the nuclear expression of HIF-1α (P = 0.003) and with the cytoplasmic expression of VEGF (P = 0.004) in gastric cancer specimens. This evidence concerns the gene HIF1A and gastric cancer.