Utilizing our previously published protocol for live infection induced antigen sensitization (Fig S1B) [10], WT, TLR2−/−, TLR4−/−, and TRIF−/− mice were infected with 5×105 IFU of CP, sensitized with HSA for three days (beginning 5 days after infection), and then challenged with HSA. The gene discussed is CP; the disease is infection.