PROM1 and cancer: Finally, we confirmed a previous report that Huh-7 cells express Prominin/AC133/CD133, an immunological marker of the “cancer stem-like” cells [34], and further found that amiloride at 0.3 mM or more down-regulated CD133 expression Huh-7 cells (Figure S2-A), resulting in suppression of the number and size of cell colony formations (Figure S2-B).