To confirm the inhibition of phosphorylated or activated STAT3 by GO-Y030 in colon cancer cells, we examined STAT3 phosphorylation (Y705) in three independent colon cancer cell lines (cells were cultured in 10% FBS) using phospho-STAT3 (Tyrosine 705) antibodies (Supplementary Figure 4). Here, STAT3 is linked to malignant colon neoplasm.