The truncated protein cannot substitute the wild type ND2 protein as frameshift altered the reading frame of ND2. Sequence variations in this gene are associated with several diseases e.g., Leigh syndrome, breast cancer, myocardial infarction, Parkinson disease, and primary congenital glaucoma (PCG) [12,26-29]. The gene discussed is MT-ND2; the disease is primary congenital glaucoma.