Given the above findings and the effect of sumoylation on transcriptional activity of Nkx2.5 [18], we asked whether CHD-linked Nkx2.5 mutants exhibited abnormal sumoylation activity and how defective sumoylation of Nkx2.5 affected cardiogenesis in vivo. The gene discussed is NKX2-5; the disease is coronary artery disorder.