TLR2.CD40 triggering also lead to downregulation of negative regulators of differentiation such as Bad, Bcl2, Xrcc4, Inpp5d etc. (Table S3).Thus, these results clearly indicated that synchronized signaling through TLR2.CD40 enhances the differentiation of RB cells into marginal zone precursors and promote their developmental process. This evidence concerns the gene CD40 and retinoblastoma.