The high selectivity of the MBD2-MBD polypeptide for methylated DNA and the high density of the oligonucleotide tiling microarrays covering all non-repetitive regions of chromosomes 21 and 22 with an average inter-probe spacing of ~10 bp allowed unbiased, high-resolution, chromosome-wide mapping of DNA methylation in the LNCaP prostate cancer cell line and the PrEC normal prostate epithelial cells in primary culture. This evidence concerns the gene MBD2 and prostate cancer.