The high selectivity of the MBD2-MBD polypeptide for methylated DNA and the high density of the oligonucleotide tiling microarrays covering all non-repetitive regions of chromosomes 21 and 22 with an average inter-probe spacing of ~10 bp allowed unbiased, high-resolution, chromosome-wide mapping of DNA methylation in the LNCaP prostate cancer cell line and the PrEC normal prostate epithelial cells in primary culture. Here, MBD2 is linked to prostate carcinoma.