Since tumor-associated ECMs are organized in parallel patterns [21,22,37], we tested whether FAP expression affects the topography of fibroblasts-derived ECMs by indirect immunofluorescence using anti-fibronectin (Figure 1) or anti-collagen I (Additional file 2, Fig. S2) antibodies on un-extracted matrices containing their original matrix-synthesizing fibroblasts [21]. The gene discussed is FN1; the disease is neoplasm.