Unfractionated PBMC preparations were used in evaluating the ability of human solid tumor cell lines to generate myeloid suppressor cells to best approximate an in vivo setting, but CD33+ suppressor cells were also generated successfully from T cell-depleted PBMC by co-culture with 4-998 osteogenic sarcoma or SCCL-MT1 head and neck squamous cell carcinoma (HNSCC) cells (Table 1). The gene discussed is CD33; the disease is head and neck squamous cell carcinoma.