Although the variation of decreased GPR30 expression did not correlate to tumor size, ERα, grade, stage, menopausal state, age and histological type, we observed a much marked decreased expression in tumor tissues from patients who were positive for lymph node metastasis compared to the tumor tissues from patients who were negative for lymph node metastasis (Table 1, Table 3 and Fig. 4) (Two sample t-test, P < 0.02, n = 29 node positive and n = 53 node negative). This evidence concerns the gene GPER1 and metastatic malignant neoplasm in the lymph nodes.