Although the variation of decreased GPR30 expression did not correlate to tumor size, ERα, grade, stage, menopausal state, age and histological type, we observed a much marked decreased expression in tumor tissues from patients who were positive for lymph node metastasis compared to the tumor tissues from patients who were negative for lymph node metastasis (Table 1, Table 3 and Fig. 4) (Two sample t-test, P < 0.02, n = 29 node positive and n = 53 node negative). The gene discussed is ESR1; the disease is neoplasm.