Our previous evaluation of Treg cells throughout the expression of the transcription factor Foxp3 in digestive clinical forms of Chagas' disease showed an increased concentration of Foxp3 T cells in IND group without megacolon compared with those patients with megacolon and non-infected individuals, suggesting that these cells possibly have an important role in the intestinal infection and may represent a mechanism used by patients presenting Chagas' disease to prevent exacerbation of the inflammation and, consequently, avoid megacolon development [21] (Box 2). This evidence concerns the gene FOXP3 and Aganglionic megacolon.