In addition, vinblastine pretreatment also limitedly reduced the percentage of CCR6−Tregs in tumor mass (Fig. 5a, p>0.05) and led to slightly elevated IFN-γ production and proliferation, as well as the expression of CD107a and Granzyme B, of CD8+T cells (Fig. 5b,c,d, p>0.05). This evidence concerns the gene CD8A and neoplasm.