To test whether transcription of Lcn2 is associated with activation of NF-κB in prostate cancer cells, we created a transgenic TC1 cell line, which carries an NF-κB reporter plasmid (TC1.pNGL) containing an enhanced green fluorescent protein (EGFP)-luciferase fusion gene [22]. Here, NFKB1 is linked to Familial prostate cancer.