By contrast, compared to the wt StAR the G221S mutant produced 30–50% pregnenolone over the observation period of 5–600 min while the L260P mutant (which is associated with severe CLAH [32]) produced pregnenolone only to the basal levels of the vector control (Figure 6B). This evidence concerns the gene STAR and congenital lipoid adrenal hyperplasia due to STAR deficency.