Interestingly, after aerosol infection, spleen cells from BCG-vaccinated/rHBHA-boosted mice produced also a modest but significant amount of IFN-γ (760 ± 312 pg/mL) (Figure 1B, gray bar), higher than spleen cells from BCG-vaccinated mice without boost (300 ± 68 pg/mL) (Figure 1B, black bar) and PBS control mice (159 ± 10 pg/mL) (Figure 1B, blank bars) (P < 0.05). This evidence concerns the gene IFNG and infection.