Although a few studies of Cx43 in human sarcoma cell lines have been reported, which include osteosarcoma, rhabdomyosarcoma, and fibrosarcoma [17–21], there has not been any studies reported on the expression of Cx43 in formalin-fixed and paraffin-embedded tissue samples of human EWS/PNET. Here, GJA1 is linked to primitive neuroectodermal tumor.