Because Pim3 also was induced by cycloheximide, which complicates interpretation of these types of experiments, we analyzed whether Myc binds the E-boxes of the Pim3 locus by performing chromatin immunoprecipitation assay (ChIP) on formaldehyde cross-linked DNA from a λ-Myc transgenic mouse B-cell lymphoma cell line, λ820, established in our laboratory [38]. The gene discussed is PIM3; the disease is B-cell non-Hodgkin lymphoma.