PDCD1 and cryohydrocytosis: With further investigation, we found (Figure 5B) that the TEM-Tregs/naive-Tregs ratio and TEM-Tregs/TCM-Tregs ratio of PD-1 MFI were significantly lower in CHC patients than healthy controls, indicating that PD-1 expression on TEM-Tregs or effector-Tregs were inhibited in relative to its correspondent naïve- or TCM-Tregs in CHC patients compared with healthy controls.