In addition, there was a significant decrease of PD-1 expression on CD127loCD25hiFoxP3+ Tregs compared with CD127+CD25lo/- (p < 0.0001 on percentage and MFI) or CD127-CD25-(p = 0.0003 on percentage and p = 0.0045 on MFI) T cell subsets in CHC patients (Figure 3B and 3C). This evidence concerns the gene PDCD1 and cryohydrocytosis.