These findings echo earlier observations of stiffness insensitivity in H-ras transformed NIH 3T3 cells [13], but contrast with the behavior of 16HBE14o- cells, which are SV40 transformed, and A549 cells, which are derived from a human lung adenocarcinoma, suggesting complexity in the molecular mechanisms by which stiffness sensitivity is lost. This evidence concerns the gene HRAS and lung adenocarcinoma.