Figure 6 shows that i.t. instillation of soluble RAGE had no effect on E. coli-mediated acute pulmonary inflammation. There was no significant difference in cell concentration in BALF between the mice receiving E. coli alone and the mice that also received mouse soluble RAGE (Figure 6A). Differential cell counts revealed an almost identical cell population in mice receiving E. coli only and those receiving E. coli plus i.t. mouse soluble RAGE (Figure 6B–C). This evidence concerns the gene AGER and inflammatory response.