Comparison to published functional screens specifically addressing cell cycle and proliferation in human cells revealed that knockdown of 12 of our hit genes produced a cell cycle defect in other systems [24], [29], [30], [31], [32] while the overexpression of CDK9, CRK, and CLK2 also increased proliferation in human hepatoma cell line SMMC7721 [11]. This evidence concerns the gene CDK9 and hepatocellular carcinoma.