The five genes identified by all the protocols were DIRAS3, CXCL6, SAMD5, CBFB (core-binding factor, beta subunit), and MEIS2. Known glaucoma causing genes, CYP1B1 (cytochrome P450, family 1, subfamily B, polypeptide 1), MYOC (myocilin), OPTN (optineurin), and WDR36 (WD repeat domain 36) were not among the genes affected by PITX2 [41]. Here, OPTN is linked to glaucoma.