To determine whether NFAT induction of TRAIL expression was regulated at the level of promoter activity, Caco-2 human colon cancer cells were transfected with plasmids encoding NFATc1, NFATc2, NFATc3, NFATc4 or control vectors pDF30 or pcDNA3.1 together with a 1371 bp TRAIL proximal promoter construct [21]. The gene discussed is NFATC4; the disease is malignant colon neoplasm.