NPTX2 and mantle cell lymphoma: To validate the methylation events identified in the MCL cell lines we investigated the methylation status of eight genes (CDH1, AHR, CDC14B, HOXA9, ROBO1, SOX9, NR2F2, and NPTX2) in 38 primary MCL using one of the previously designed amplicons for each gene (Figure 2A) that showed specific hypermethylation in at least two MCL cell lines (Figure 1).