We also examined the expression of HOXD9 mRNA in four glioma cell lines, U87, SK-MG-1, KNS-42 and KNS-81 and found higher expression of HOXD9 mRNA compared with that in normal human astrocytes (33-, 32.5-, 52.8-, and 57.5-fold higher, respectively; Figure 1D). This evidence concerns the gene HOXD9 and glioma.