This relationship was reflected in associations between Wnt5a methylation and MSI-associated tumour characteristics, including immunohistochemical deficiency of at least one mismatch repair protein (OR=7.2, 95% CI=4.3–12.2, P<10−12), MLH1 methylation (OR=14.9, 95% CI=7.8–28.5, P<10−16), proximal location (OR=3.6, 95% CI=2.3–5.6, P<10−7), mucinous histological type (OR=2.0, 95% CI=1.1–3.7, P=0.03), and female gender (OR=2.5, 95% CI=1.6–3.8, P<10−4) (Table 2). This evidence concerns the gene MLH1 and neoplasm.