Wnt5a methylation was similarly associated with MSI tumours (OR=5.0, 95% CI=3.0–8.4, P<10−18) and the same set of MSI-associated characteristics: MMR IHC deficiency (OR=6.2, 95% CI=6.6–10.7, P<10−9), MLH1 methylation (OR=36.7, 95% CI=10.3–130.4, P<10−10), proximal location (OR=3.2, 95% CI=2.1–4.9, P<10−7), mucinous histological type (OR=1.9, 95% CI=1.1–3.2, P=0.02), and female gender (OR=1.6, 95% CI=1.1–2.3, P=0.03) (Table 2). Here, MLH1 is linked to neoplasm.