Although shedding of the ADAM10 ectodomain was reported to be increased by phorbol ester in SH-SY5Y neuroblastoma cells [20], treatment of HEK-M3 cells with carbachol had no effect on the generation of the ADAM10 CTF, either in the presence or absence of dyn I K44A (Figure 5). This evidence concerns the gene ADAM10 and neuroblastoma.