Although the histological and immunohistochemical results indicated that all PLGA formulations coated with poloxamer 188 were able to transport doxorubicin to the tumour, the Dox-Lecithin-PLGA/HSA produced the most considerable effect on all investigated histological parameters, i.e. the tumour size, proliferation index, GFAP expression, extent of necrotic areas, and vessel density, as well as VEGF expression. This evidence concerns the gene ALB and neoplasm.