To investigate the potential interactions between MYC overexpression and RB loss of function in HCC, we bred conditional mutant Rblox/lox mice [41] with LAP-tTA TRE-MYC mice, in which expression of a human MYC cDNA can be induced specifically in the liver [32], [42] (Figure 1A). Here, RB1 is linked to hepatocellular carcinoma.