We used immunized sera from two subjects who demonstrated both evidence ofpost-vaccination cellular immune responses and prolonged disease-free survival(DFS) and overall survival (OS) in a phase II study of a GM-CSF secreting wholecell PDA vaccine [4] to screen whole cell extracts from the PDA vaccinecell lines which served as the proteome. The gene discussed is CSF2; the disease is Patent ductus arteriosus.