EDN1 and Alzheimer disease: Indeed, our results relating to the AD state in vitro model (represented by LnCaP cells) support that the latter have lower secreted levels of ET-1, also shown by Nelson et al. [13] and Grant et al. [33], which could be the combined outcome of both NEP-mediated degradation but also reduced ET-1 production due to decreased ECE-1 expression [34].