The observation that WSX1-positive tumors induce T cell death was further confirmed via trypan blue exclusion assay, in which splenocytes were co-cultured with LLC-GFP or LLC-WSX1 and the viable splenocytes were reduced in the presence of WSX1-positive tumor cells as compared to the cognate control cells (Figure 7b). This evidence concerns the gene IL27RA and neoplasm.