Studies comparing the total cellular (cells were permeabilized) or membrane expression of NKG2D in the splenocytes of mice bearing LLC-GFP or LLC-WSX1 revealed that extracellular and total cellular NKG2D expression was increased rather than decreased by WSX1 (Figure 3c) suggesting that internalization of NKG2D is not the mechanism that explains the pro-tumor properties of WSX1 in LLC model. This evidence concerns the gene IL27RA and neoplasm.