STX1A and hemolytic-uremic syndrome: The objectives of the present study were 1) to develop a standard antibody ELISA to detect anti-Stx2 B subunit, and a WB assay against the whole Stx2 and Stx1 proteins; 2) to correlate the results from anti-Stx2B ELISA with those from anti-Stx2 WB and to validate them to be used in our population; and 3) to study the presence of antibodies against Stx2 A and B subunits in normal healthy children and HUS patients from Argentina.