VEGF-A secretion by breast cancer cell lines MDA-MB-468, T-47D, MCF-7, HBL-100, and MW1 were about 2.3 to 37.3 ng/ml for 106 cells for 48 hours, which was higher above the biological activity of VEGF-A (1–50 pg/ml),37 indicating the role of VEGF in breast cancer progression. This evidence concerns the gene VEGFA and breast carcinoma.