IFNG and neoplasm: Bone marrow-derived DC were cultured for 16 h in the presence of LPS and IFNγ, with either live B16 cells and the caspase inhibitor zVAD to avoid caspase-mediated apoptosis (DC-B16 zVAD), or apoptotic B16 cells (DC-B16γ) (Fig. 1B), or tumor specific MHC class I-restricted peptides (gp10025–33 and TRP2181–188, DC-peptides), or medium alone (DC).