Consistent with theseobservations, the ALS-linked FUS variants—H517Q, R521C, andR521G—aggregated with very similar kinetics to WT in pure proteinaggregation assays, although aggregation was slightly retarded in these mutants(Figure 9E).Collectively, these data suggest that this set of ALS-linked FUS mutations,clustered in the extreme C-terminal region of FUS, do not promote FUSaggregation per se. The gene discussed is FUS; the disease is amyotrophic lateral sclerosis.