Our aims were at first to determinate expression profile of GSK3ß and its regulatory genes (Wnt ligands 1, 3a, 5a, Frizzled Receptor 1 and 2, sFRP-1 and Axin1) and quantify protein level changes and phosphorylation status in MCT-induced PAH rat lungs and in isolated PASMCs. Here, SFRP1 is linked to pulmonary arterial hypertension.