In this study, overexpression of Pyk2 in Hep3B cells resulted in a down-regulation of E-cadherin expression while suppression of Pyk2 by PRNK in MHCC97L cells significantly up-regulated the expression of E-cadherin, suggesting the important mechanism of Pyk2 on the regulation of adhesiveness of HCC cell via regulation of E-cadherin expression. This evidence concerns the gene PTK2B and hepatocellular carcinoma.