Forced overexpression of Pyk2 in the non-metastatic HCC cell line Hep3B (Hep3B-Pyk2 cells) enhanced the formation of lamellipodia and membrane ruffles upon stimulation by PDGF-BB or LPA compared to control Hep3B-vector cells (Fig. 1A). Here, PTK2B is linked to hepatocellular carcinoma.